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KMID : 0380020060210050341
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 5 p.341 ~ p.346
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The Early Detection of the Gram Negative Bacteria using Sonification and Fluorescent Dye in the Field
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Ha Yeon-Chul
Choi Ki-Bong
Choi Jung-do
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Abstract
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This study was carried out to establish the optimum condition for cell disruption with a sonificator in the detection of the gram negative bacteria, E. coli for the purpose of developing automatic fluorometer. The efficiency of sonification on the E. coli disruption was greatly dependent on the diameter of sonificator probe tip. The larger sonificator probe diameter showed greater disruption effect. Sonificator probe of 13 mm diameter was the most efficient one for E. coli when sonificated for 20 seconds. The efficiency of the E. coli disruption differed greatly according to the depth of sonificator probe tip sank in the sample solution. The shorter the distance between probe tip end and the bottom of the container, the higher the disruption efficiency. The detection limit of E. coli was 5 × 105 CFU/㎖ when sample was sonificated for 20 seconds with a sonificator probe of 13 mm diameter.
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KEYWORD
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Sonification, disruption, fluorometer, detection
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